Quantitative spatial and temporal analysis of fluorescein angiography dynamics in the eye

Abstract

Purpose: We describe a novel approach to analyze fluorescein angiography to investigate fluorescein flow dynamics in the rat posterior retina as well as identify abnormal areas following laser photocoagulation.Conclusions: This simple registration and analysis provides a way to monitor the size of vascular injury, to highlight areas of subtle vascular leakage and to quantify vascular dynamics not possible using current fluorescein angiography approaches. This can be applied in both laboratory and clinical settings for in vivo dynamic fluorescent imaging of vasculature.Methods: Experiments were undertaken in adult Long Evans rats. Using a rodent retinal camera, videos were acquired at 30 fram..